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Autofluorescence imaging cancer. These techniques are broadly discussed in this review.
Autofluorescence imaging cancer. The function of a T cell depends on its subtype and activation state. The best way to assume normal parathyroid gland (PTG) function is to preserve them in situ, undamaged. A Jan 1, 2005 · The term “autofluorescence” is used to distinguish the intrinsic fluorescence of cells and tissues from the fluorescence obtained by treating specimens with exogenous fluorescent markers that binds cell and tissue structures. This method could inform new immunotherapy approaches for cancer Mar 29, 2022 · Nonlinear optical imaging can provide abundant cancer invasion-associated information (by SHG) and redox-ratio information (by NADH and FAD) for comprehensive pathological analyses of cancers 4. We develop an NIR autofluorescence imaging system combination with polarization technique to explore its potential for cancer diagnosis and detection. Mar 30, 2023 · Autofluorescence, the natural emission of light by biological structures, is a widely occurring phenomenon in cells and tissues. Near-infrared autofluorescence imaging of tissues under long-wavelength laser excitation in the green and red spectral region complemented by cross-polarized elastic light scattering was explored for cancer detection. It’s also a powerful tool for monitoring endogenous fluorophores and biological conditions. Mar 1, 2017 · Optical metabolic imaging (OMI) is two-photon imaging of the autofluorescence intensities and lifetimes of NAD (P)H and FAD. Collagen, which fluoresces in the green wavelength range, is one of the major sources of tissue autofluorescence (AF) Abstract Introduction: Near-infrared autofluorescence imaging (NIFI) can be used to identify parathyroid gland (PG) during surgery. Autofluorescence is defined as the fluorescence of naturally occurring substances, such as chlorophyll and collagen, that is observed in most plant and animal tissues when excited by ultraviolet light. Mar 21, 2025 · Intraoperative misidentification or vascular injury to the parathyroid glands can lead to hypoparathyroidism and hypocalcemia, resulting in serious postoperative complications. , will explain the use of multiphoton autofluorescence imaging of NAD (P)H and FAD, co-enzymes of metabolism, to monitor T-cell activation and function. aWe also assess … Nov 15, 2021 · Metabolic perturbations by cyanide verify autofluorescence imaging protocols to detect metabolic changes within cells. It’s caused by endogenous molecular components exhibiting the ability to fluoresce. Mar 20, 2025 · Cancer Stem Cells (CSCs) can drive drug resistance, tumor recurrence, and metastasis. Methods: Studies adopting autofluorescence devices, evaluating the efficacy of com‐prehensive oral examination and optical fluorescence imaging in detection, visualisa‐tion or management of oral squamous cell carcinoma or oral potentially malignant disorders, as well as discriminating oral epithelial dysplasia from other mucosal le‐sions, were included in the literature search across Background: Detection of nonsmall cell lung cancer at the intraepithelial stage is believed to improve cure rates. Colon tissues (normal vs. Sep 1, 2016 · Learn about fundus autofluorescence imaging, the origin of autofluorescence, common imaging systems, and characteristic FAF findings in retinal diseases, including a preview of the second part of the series. 1), and the tongue (Fig. Fundus autofluorescence (FAF) is a relatively new imaging technique that can be used to study retinal diseases. Oct 9, 2018 · Objectives Autofluorescence imaging is gaining popularity as an adjunctive test for oral potentially malignant disorders (OPMD). F … Nov 24, 2017 · Here, we perform autofluorescence imaging of human lung cancer invading a human functional airway using an in vitro model of Non Small Cell Lung Cancer which combines a reconstituted human airway Dec 29, 2023 · Hypoparathyroidism is the most frequent complication in thyroid surgery. The aim is to evaluate the accuracy of autofluorescence for screening oral cancer in comparison with toluidine blue staining and clinical examination. The determined diagnostic algorithms may be an effective diagnostic solution in the diagnosis of skin lesions of this nature. This article reviews the progress of the clinical application of AFI in the diagnosis of various gastrointestinal tumors. Wong, Yan Zhang, Tony K Feb 14, 2025 · Autofluorescence from endogenous biomolecules presents a significant challenge in immunofluorescence microscopy, often severely hindering the detection of specific fluorescence signals. While Mar 18, 2018 · Optical imaging in the head and neck has been reported using autofluorescence imaging, targeted fluorescence imaging, high-resolution microendoscopy, narrow band imaging and the Raman spectroscopy. Five hundred and twenty three NIFI Among these, autofluorescence based diagnostic techniques are rapidly emerging as a powerful tool. Through quantitative analysis on the target area of autofluorescence bronchoscopy image, determine the optimal Due to the high sensitivity of autofluorescence examination, it has a vital role in determining high-risk oral lesions (precancerous) and oral cancer in mass screening programs for the cancer-prone population. In the present study, autofluorescence images of cervical cell were assessed for early cervical cancer risk prediction. Cancerous lesions display loss of autofluorescence due to malignant changes in epithelium and subepithelial stroma. Dentists play an important role in the early detection of oral cancer. Near-infrared (NIR) fluorescence imaging is a promising new technique that uses non-specific fluorescent agents and targeted fluorescent tracers combined with a dedicated camera to better navigate and visualize tumors. This article will demonstrate the technique of autofluorescence imaging of NAD (P)H and FAD for measuring cellular metabolism. The AFI image of colorectal cancer is different according to its macroscopic type. et al. Jul 6, 2016 · In this paper, autofluorescent gelatin nanoparticles were synthesized as matrix metalloproteinase (MMP) responsive probes for cancer cell imaging. However, these are primarily intended for use in healthcare facilities in industrialized countries. Therefore, we investigated the potential of a commercial confocal laser scanning endomicroscopy (CLE) system for clinical imaging of brain tumors. M. Bronchoscopic findings of an invasive squamous cell carcinoma of the central airway on autofluorescence imaging (A), white light bronchoscopy (B) and narrow band imaging (C). This technique has greater sensitivity and specificity, in assessment of tumor margins, than WLB alone. Measuring mitochondrial activity is useful to characterize cancer metabolism patterns, to identify metabolic vulnerabilities and to identify new drug targets. Abstract Nasopharyngeal cancer (NPC) is an endemic with high incidence in Southern China and Southeast Asia countries. Dec 1, 2017 · We assessed the ability of fluorescence lifetime imaging microscopy (FLIM) for differentiating human lung cancer and normal tissues with the autofluorescence, and also elucidated the mechanism in tissue studies and cell studies. Dec 22, 2023 · Low-cost optical imaging technologies have the potential to reduce inequalities in health care by improving the detection of precancer or early cancer and enabling more effective and less invasive Nov 18, 2016 · A recent study reports that autofluorescence is a promising and noninvasive tool for the detection of oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMDs). cancer) from patients who underwent colonoscopy or surgical resections were used for this study. Therefore, measurements of cellular-level tumor heterogeneity could enable improved anti-cancer treatment regimens. This allows precise collection of molecularly specific data Jan 7, 2020 · An original method based on scar autofluorescence assessment can be applied to identify the recurrence of skin cancer in postoperative scars. The experiments were conducted on the autofluorescence images captured by clinicians/dental specialists from the Oral Cancer Research Co-ordination Centre (OCRCC), Faculty of Dentistry, at the University of Malaya (UM). Autofluorescence imaging was performed using a modified fluorescence endoscopy system from Xillix Technologies … Autofluorescence examination of oral tissues using the VELscope has been suggested as an adjunctive tool for cancer detection and diagnosis. Quantitative imaging of intensity-based contrast is Our results confirm that AFI videobronchoscopy significantly improves the assessment of central lung cancer extension and influences the therapeutic strategy. This produces a stack of images where each matched pixel contains information about the sample's spectral properties at that location. The proposed diagnostic parameter is based on a reduction in scar autofluorescence, evaluated in the green spectral Nov 23, 2010 · We investigated whether the extent of atrophic fundic gastritis diagnosed by autofluorescence imaging (AFI) videoendoscopy is predictive of development of metachronous gastric cancer after H. Autofluorescence is the natural fluorescence of biological structures such as mitochondria and lysosomes, in contrast to fluorescence originating from artificially added fluorescent markers (fluorophores). The tissue imaging including NIR white light images, autofluorescence (AF) images and FPI images (parallel-, and Jan 4, 2020 · Background Many recent studies have reported that autofluorescence bronchoscopy (AFB) has a superior sensitivity and decreased specificity in the diagnosis of bronchial cancers when compared with white-light bronchoscopy (WLB). Jun 30, 2025 · Autofluorescence is a term used to describe the background fluorescence observed in biological samples that does not result from staining with a specific fluorescent probe. Feb 3, 2025 · Background Hypoparathyroidism is the most common complication of thyroid surgery. pylori eradication in patients treated with endoscopic submucosal dissection (ESD) for EGC. We specifically analyzed the diagnostic performances of autofluorescence imaging video bronchoscopy (AFI) performed with the Evis Lucera Spectrum from Olympus, which is Jul 27, 2020 · The function of a T cell depends on its subtype and activation state. Materials and methods In total, 126 patients suspected to have mucosal disorders at the Division of Oral and Maxillofacial Surgery Apr 1, 2024 · White light, autofluorescence and narrow-band imaging bronchoscopy for diagnosing airway pre-cancerous and early cancer lesions: a systematic review and meta-analysis Abstract Bronchoscopy technology is a desirable method for detecting lung cancers arising in the central airways. Narrow-band imaging indicated that subepithelial vascular regularity and distribution patterns were within normal limits. Autofluorescence imaging and spectroscopy is a non-invasive, label-free, real-time technique for cancer detection. Practical hints for the use of fluorescent probes and newly developed sensors for imaging ΔΨm, ATP and ROS are described. Sep 13, 2018 · Fluorescence lifetime imaging microscopy of NADH autofluorescence offers a marker-free readout of the mitochondrial function of cells in their natural microenvironment and allows different pools of NADH to be distinguished within a cell. Cells and tissues are viewed through optical filters that allow the passage of long wavelength red and green autofluorescence. This clinical study is a first attempt to use autofluorescence for recurrence diagnosis of skin cancer in post-operative scars. There were more than 270,000 new cases of oral cancer reported in 2002 (1). Autofluorescence imaging is increasingly used to noninvasively identify neoplastic oral cavity lesions. It can mask specific fluorescence but also provides insights into tissue morphology. Autofluorescence Imaging and Virtual Histological Staining of Human Prostate Sections for Cancer Diagnosis Mingxuan Si, Weixing Dai, Ivy H. The early Jun 1, 2020 · Can fluorescence and autofluorescence imaging be useful in diagnosis of basal cell cancer? Proposition of algorithms Sebastian Kwiatek a , Aleksandra Kawczyk-Krupka b , Ewa Mańka c , Grzegorz Cieślar b , Aleksander Sieroń a d , Agata Stanek b Show more Add to Mendeley Apr 4, 2025 · New non-destructive tools with single-cell resolution are needed to reliably assess B cell and NK cell function for applications including adoptive cell therapy and immune profiling. Therefore, two photon NADH auto-fluorescence imaging can be implemented to illustrate vascular supply regions and localization of functional micro-regional cortical hypoxia with a spatial resolution in the micro-meter range. Here, a widefield deep‐ultraviolet Abstract Autofluorescence imaging is an emerging method for detection of cancer associated risks, depending on the variation in autofluorescence property of normal and cancer cells. 2) are observed. Jun 28, 2024 · Label-free autofluorescence lifetime is a unique feature of the inherent fluorescence signals emitted by natural fluorophores in biological samples. Methods: Five hundred and twenty three NIFI images were selected. 24, 16 Conclusion Apr 2, 2009 · Two-photon autofluorescence lifetime imaging reveals heterogeneous environment of the intracellular NADH, which is distinct in breast cancer and normal cells. Mar 8, 2024 · Abstract Introduction Near-infrared autofluorescence imaging (NIFI) can be used to identify parathyroid gland (PG) during surgery. Jun 26, 2023 · Combining autofluorescence with novel techniques and tools such as fluorescence lifetime imaging microscopy (FLIM) and hyperspectral imaging (HSI) with model-free analysis of phasor plots has revolutionized the understanding of molecular processes such as cellular metabolism. Autofluorescence is the term given to describe the natural fluorescence that occurs in cells. Jul 21, 2011 · This study aimed to assess and compare effectiveness of Autofluorescence imaging (AFI) in diagnosis of early gastric cancer (EGC) between experienced and less experienced endoscopists. Summarizes clinical trials to evaluate the efficacy of autofluorescence imaging ( VELscope) in detecting oral cancer and oral potentially malignant disorders. Several different pathologies can be detected. Autofluorescence bronchoscopy shows good sensitivity and poor specificity in detecting dysplasia and cancer of the bronchus. Understanding the biological basis of autofluorescence imaging for oral cancer detection: high resolution fluorescence microscopy in viable tissue. Oct 12, 2020 · HPIL is a novel system approach based on the textural pattern of OML and OPMDs (anomalous regions) to differentiate them from standard regions of the oral cavity by using autofluorescence imaging. The emergence of newly developed optical instrumentation in addition to the discovery of new fluorescence biomolecules have contributed to a refined management of diseases and tumors, especially in the management of upper aerodigestive tract tumors Feb 1, 2005 · It has been claimed that autofluorescence spectroscopy and imaging can assist in oral oncology for the detection and classification of lesions. New bronchoscopic technologies, including white light videobroncho-scopy (WLB), autofluorescence imaging (AFI), and narrow band imaging (NBI), are aiming to diagnose airway neoplasia at a preinvasive stage. Sep 9, 2024 · Incorporating AI helps enhance fluorescence imaging and is poised to bring major innovations to surgical guidance, thereby realizing precision cancer surgery. Early diagnosis significantly improves survival rates and patient outcomes, yet in resource-limited settings, the scarcity of medical resources often leads to late-stage diagnosis. Lung cancer is one of the most common cancers, with high mortality rate worldwide. This study aimed to evaluate OPMDs with autofluorescence imaging, reflectance spectroscopy and vital staining technique and compare with histopathology. However, it has a limitation in diagnostic specificity due to high false positive rates. Autofluorescence bronchoscopy (AFB) is a newly developed technology that exploits the difference in autofluorescence intensity between normal and tumorous tissues to detect Abstract Both narrow band imaging (NBI) and autofluorescence imaging (AFI) are new techniques for the assessment of lung cancer. In particular, autofluorescence imaging has emerged as a promising adjunctive technique to improve early identification of oral premalignant lesions. Most early cancers and precancerous lesions are not visible on conventional white-light bronchoscopy (WLB). This study aimed to determine the diagnostic value of VELscope in a large prospective study of 517 patients with oral potentially malignant disorders (OPMD). Fluorescence was induced by blue light at 442 nm and captured by an image Near‐infrared autofluorescence imaging (NIFI) can be used to identify parathyroid gland (PG) during surgery. Discover how fundus autofluorescence enhances retinal disease diagnosis, aids in macular degeneration screening, and supports early detection of diabetic retinopathy with non-invasive imaging. It provides information on retinal metabolism and health. Typically, healthy tissue appears green (emits fluorescence) while abnormal tissue appears dark [13]. Abstract Autofluorescence imaging is an emerging method for detection of cancer associated risks, depending on the variation in autofluorescence property of normal and cancer cells. Autofluorescence and fluorescence imaging allow assessment of the extent of neoplastic infiltration and distinguish types of skin lesions with BCC. A 15-patient testing group was used to compare FLIM results with traditional histopathology diagnosis. Due to the invisible margins of changes on the skin, determining skin cancer’s boundaries can be difficult without the use of modern imaging methods [1,5]. Cellular autofluorescence can be due to the presence of collagen and elastin, cyclic ring compounds such as NADPH and riboflavin, aromatic amino acids and cellular organelles such as mitochondria and lysosomes. Peculiar AF alterations can help Oct 1, 2021 · An overview of the current clinical status of optical imaging in head and neck cancer with a focus on Narrow Band imaging and fluorescence optical imaging. Several types of AFB system have been designed and developed. Apr 28, 2015 · Among these, autofluorescence based diagnostic techniques are emerging as one of the most potential and dynamic tool in the early diagnosis of oral cancer. Conclusion: PpIX autofluorescence clearly differentiated the tumor from adjacent normal tissues. Quantitative imaging of intensity-based contrast is notoriously difficult and spectrally resolved imag … May 20, 2025 · Autofluorescence imaging with virtual histological staining has shown promising potential to improve the efficiency of postoperative histopathology workflow. Dec 1, 2024 · A wide-field (10 × 10 cm 2) near-infrared autofluorescence (NIRAF) imaging subsystem was developed for gross stratification of breast tissue types based on higher NIRAF intensity associated with breast cancer, followed by projection of NIRAF-identified breast tumor margins onto the tissue of interest with a compact projector. Improving the diagnostic accuracy of these techniques requires a better under-standing of the biological basis for optical changes associated with neoplastic transformation in oral tissue. As such, it can be used to interrogate tissue autofluorescence. On the other hand the reliable use of autofluorescence in cancer diagnostics is depending upon a learning curve which means that it requires a lot of experience with the device. Patients undergoing total thyroidectomy with central neck dissection from 2018 to 2022 were included. Fresh samples were Optical imaging of tissue autofluorescence has the potential to provide rapid label-free screening and detection of surface tumors for clinical applications, including when combined with endoscopy. The 2P intensity (A, C) and FLIM (B, D) images of breast normal (A, B) and cancerous (C, D) cells reveal different autofluorescence lifetime distribution as shown in the corresponding Dec 26, 2024 · Purpose Analysis of autofluorescence holds promise for brain tumor delineation and diagnosis. Optical imaging, especially fluorescent microscopy, is one of the most valuable Dec 28, 2016 · Lung cancer is one of the most common cancers, with high mortality rate worldwide. Cells have a natural level of fluorescence, called autofluorescence, which can be a problem in flow cytometry data analysis. Ma … A visual deep learning model to localize parathyroid- specific autofluorescence on near- infrared imaging: localization of parathyroid autofluorescence with deep learning. Mar 1, 2011 · Optical imaging systems are robust, portable, relatively inexpensive, and have proven utility in detecting precancerous lesions in the lung, esophagus, colon, oral cavity and cervix. Computed tomography and MRI can be used to assess for tumor extent in relation to intra- and extra-laryngeal structures, especially paraglottic and pre-epiglottic space involvement as well Nov 1, 2019 · As a result, several non-invasive diagnostic adjuncts, including autofluorescence imaging, have been developed with the aim to aid in the early detection of oral cancer, which may help practitioners better evaluate OPMD before definitive biopsy [5]. Apr 22, 2010 · In this article, we evaluate a simple, low-cost optical imaging system that is designed for early detection of oral cancer in resource limited settings. Differences in autofluorescence (fluorescence without photodynamic drugs) between normal and malignant tissues offer new possibilities in detecting and localizing early laryngeal carcinoma. We describe the use of light-induced endogenous fluorescence (autofluorescence) in identifying preinvasive and occult carcinomas in ex vivo samples of human fallopian tube (FT) epithelium. Near-infrared fluorescence (NIRF) imaging is an attractive modality for early cancer detection with high sensitivity and multi-detection capability. It aids us in the Sep 1, 2021 · We aim to evaluate the diagnostic value of autofluorescence laryngoscope (AFL) in early laryngeal carcinoma and precancerous lesions. The results of PpIX autofluorescence imaging and 5-ALA-induced fluorescence imaging were identical. Endoscopy is needed to assess for vocal fold function that directly affects staging. OMI is currently under investigation for drug screening in 3D tumor cultures, including breast cancer. Consequently, early detection and diagnosis based on morphologic changes to the microvessels are crucial. Autofluorescence serves as a valuable indicator of cellular processes and states, aiding in the identification of cell populations and assessment of cell health and function in conventional flow cytometry. Integrating artificial intelligence (AI) with smartphone-based imaging systems offers a promising solution by providing Jul 1, 2022 · There is accelerated development for fluorescence and autofluorescence imaging guided liver surgery. Dec 22, 2022 · At this juncture, autofluorescence and narrow-band imaging have resurfaced in the medicine arena in parallel with current technology advancement. However, autofluorescence imaging (AFI) revealed a magenta-colored area on the bronchial epithelium corresponding to the initial lesion site. Laser-induced fluorescence endoscopy (LIFE) system (Xillix Technologies, Vancouver, Canada), consists of a helium-cadmium laser May 10, 2023 · Introduction: The intrinsic autofluorescence of biological tissues interferes with the detection of fluorophores administered for fluorescence guidance, an emerging auxiliary technique in oncological surgery. Patients with OPMD undergoing surveillance for the development of oral cancer were examined using AFI at successive clinic visits. Biopsy is the gold standard for a definitive diagnosis but is invasive and painful, while fluorescence visualization is non-invasive, convenient, and real-time, and examinations can be repeated using optical instruments. In this study, lung tissue sections excised from patients were detected by laser scan confocal microscopy and spectroscopy. Skala will demonstrate how this is a powerful method for label-free, nondestructive monitoring of T-cell metabolism within single cells. Head and neck cancer, including cancers of the oral cavity, currently ranks as the sixth most common malignancy in the world. Fluorescence imaging system Tissue autofluorescence was studied using a fluorescence imaging and point spectroscopy prototype designed by the Division of Biophysics & Bioimaging, Ontario Cancer Institute, University Health Network, Ontario, Canada (30). Methods: The image enhancement system was introduced to 164 patients who underwent laparoscopic gastric cancer surgery as the AF group. The described dual-modality, dual-view, point-of-care oral cancer screening device, developed for high-risk populations in remote regions with limited infrastructure, implements autofluorescence imaging (AFI) and white light imaging (WLI) on a Oct 1, 2021 · Loss of tissue autofluorescence has been associated with malignant and pre-malignant lesions. Here, autofluorescence lifetime imaging of the metabolic coenzymes NADH and FAD was combined with multivariate analysis and machine learning classification models to develop a label-free Jul 15, 2016 · Zaric, B. Mar 30, 2023 · Autofluorescence imaging permits label-free cell type identification in live airway tissue and reveals the dynamic formation of airway secretory cell associated antigen passages. Keywords: Fluorescence imaging device, leukoplakia, oral squamous cell carcinoma, oral submucous fibrosis I NTRODUCTION Differences in autofluorescence (fluorescence without photodynamic drugs) between normal and malignant tissues offer new possibilities in detecting and localizing early laryngeal carcinoma. Aug 4, 2024 · Light sheet fluorescence microscopy (LSFM) is a transformative imaging method that enables the visualization of non-dissected specimen in real-time 3D. Nov 1, 2024 · This study extends our previous work on virtual staining from autofluorescence, demonstrates the clinical utility of this technology for prostate cancer, and exemplifies a rigorous standard of qualitative and quantitative evaluation for digital pathology. Dec 2, 2020 · Label-free metabolic imaging and microscale culture technologies enable monitoring of single-cell macrophage metabolism, migration, and function in the 3D tumor microenvironment. Near-infrared (NIR) fluorescence cancer imaging is a growing field for both preclinical and clinical application to the clinical management for cancer patients due to its advantageous features, including a high spatial resolution, portability, In this webinar, Melissa Skala, Ph. We report results of a clinical study conducted at Tata Memorial Hospital (TMH) in Mumbai, India using this system as a tool to improve detection of oral cancer and its precursors. May 15, 2020 · We report the development of an instrument employing Single Photon Avalanche Diode (SPAD) arrays to realize real-time multispectral autofluorescence lifetime imaging at a macroscopic scale using Sep 5, 2021 · Abstract Autofluorescence imaging is an emerging method for detection of cancer associated risks, depending on the variation in autofluorescence property of normal and cancer cells. We investigated whether the extent of atrophic fundic gastritis diagnosed by autofluorescence imaging (AFI) videoendoscopy is predictive of development of metachronous gastric cancer after H. In this paper we present an image classification approach based on autofluorescence and white light images using deep learning methods. Jan 30, 2024 · The current review focuses on the latest advances in the improvement and application of fluorescence imaging technology. Oral mucosal disorders detected by autofluorescence were compared with those detected using toluidine blue staining. Objective: To assess the surgical outcomes of NIRAF-imaging utility in thyroid surgery. Oct 2, 2024 · The term autofluorescence refers to any background fluorescence that occurs in cells or tissues that have not been stained with a specific fluorescent marker. Keywords: Autofluorescence imaging; autofluorescence spectroscopy; autofluorescence visualization; early diagnosis; noninvasive; oral cancer; porphyrins; visually enhanced lesion scope. The common compounds that give rise to this fluorescence signal include cyclic ring compounds like NAD (P)H, Collagen, and Riboflavin, as well as aromatic amino acids including tyrosine, tryptophan, phenylalanine. NADH in tissue boundaries show a definitive cylindrical geometry and circumscribe coaxial central blood vessels. They can use various tools and techniques to aid in detection, including visual examination, palpation, and imaging technologies such as autofluorescence visualisation. When excited with radiation of suitable wavelength, some cell and tissue components behave as endogenous fluorophores: they pass to an excited state and then decay to Methods: Studies adopting autofluorescence devices, evaluating the efficacy of comprehensive oral examination and optical fluorescence imaging in detection, visualisation or management of oral squamous cell carcinoma or oral potentially malignant disorders, as well as discriminating oral epithelial dysplasia from other mucosal lesions, were Sep 10, 2023 · Autofluorescence is a natural phenomenon by which a fluorophore is excited by a certain wavelength of light, causing it to re-emit this light at a longer wavelength. Due to convenient modification by conjugating with moieties of interests, NIRF probes are ideal High- Definition and Autofluorescence Bronchoscopic Imaging for Evaluating Epithelial Changes in Squamous Cell Lung Cancer After Neoadjuvant Immunochemotherapy: A Case Report Dec 23, 2023 · Beyond pairing multiple optical imaging modalities to obtain broader metabolic or structural context, there are opportunities to leverage the noninvasive nature of autofluorescence imaging for direct comparisons with high-content assays. Several optical diagnostic methods such as diffuse optical tomography, wide-field autofluorescence imaging, confocal microscopy, and optical coherence tomography, are currently under development to enable earlier detection of cancer. Aug 31, 2020 · The aim is to evaluate the accuracy of autofluorescence for screening oral cancer in comparison with toluidine blue staining and clinical examination. Autofluorescence imaging was performed using a modified fluorescence endoscopy system from Xillix Technologies (Richmond, Canada). Objective To assess the surgical outcomes of NIRAF-imaging utility in thyroid surgery Mar 24, 2024 · Background: Autofluorescence photography can detect specific light-tissue interactions and record important pathophysiological changes associated with nonmelanoma skin cancer (NMSC), which has been ascribed to the fluorescence of an aromatic amino acid, tryptophan. Abstract Oral cancer is a growing health issue in a number of low- and middle-income countries (LMIC), particularly in South and Southeast Asia. The current clinical prospects of various optical imaging technologies Oct 12, 2020 · The autofluorescence images were captured with a Cannon D620 using a VELscope ® device by clinicians/dental specialists. Optical metabolic imaging (OMI) is a label-free method that measures the autofluorescence intensity and lifetime of t … Oct 1, 2021 · Loss of tissue autofluorescence has been associated with malignant and pre-malignant lesions. Near-infrared autofluorescence (NIRAF)-imaging has been introduced as a potentially useful adjunct in thyroid surgery. 1 Fundus autofluorescence is an in vivo imaging tool that enables non-invasive metabolic mapping of natural and pathological fluorophores Table 2 continue. Sep 1, 2004 · Over the last 15 years, optical spectroscopy and imaging has been intensively studied to improve the detection and localization of early lung cancer. The major aim of this study was to investigate whether the combination of these two techniques improve sensitivity and specificity in the assessment of lung cancer extension. The integration of autofluorescence and videobronchoscopy provides clear images of normal and pathologically altered bronchial mucosa. Oct 28, 2009 · Autofluorescence imaging is used widely for diagnostic evaluation of various epithelial malignancies. Therefore, AFI may be a useful adjunct to endoscopy to identify patients at high risk of developing gastric cancer. Its authors also note that using autofluorescence technology as an adjunctive imaging tool may be most reliable in the hands of oral specialists, but the use of promising Mar 1, 2021 · Graphical abstract Autofluorescence and fluorescent probes allow the early detection of oral cancer through the loss of tissue autofluorescence or through the selective accumulation of fluorescent molecules in tumor cells after addition of fluorescent probes. Autofluorescence bronchoscopy (AFB) is the most successfully developed technique and has significantly improved the detection sensitivity of early lung cancer. Carcinoma of unknown primary site We formulated a key question ‘What is the clinical effec-tiveness of VELscope in detecting oral potentially malig-nant disorders?’ to address the role of autofluorescence imaging in early detection of oral cancer among adults during routine oral examination. Protruded lesion, which is the most common macroscopic type of colorectal neoplasms, is revealed as magenta protruded lesion. OMI, which exploits the endogenous fluorescence from the metabolic coenzymes NAD(P)H and FAD, is sensitive to changes in cell metabolism … Abstract With the goal to screen high-risk populations for oral cancer in low- and middle-income countries (LMICs), we have developed a low-cost, portable, easy to use smartphone-based intraoral dual-modality imaging platform. The fluorescence method is an innovative technique used by pathologists for examining body mucosa, and for the abnormalities tissue screening, potentially leading to the earlier discovery of pre-cancer, cancer or other disease processes. Apr 26, 2001 · Detection of early gastric cancer by a real-time autofluorescence imaging system Masahiko Kobayashi a , Hisao Tajiri b , Eiji Seike a , Masanori Shitaya a , Shigetaka Tounou a , Masafumi Mine a , Kenichi Oba a Show more Add to Mendeley Hyperspectral and multispectral imaging of cell and tissue autofluorescence is an emerging technology in which fluorescence imaging is applied to biological materials across multiple spectral channels. Here, we show that the imaging of autofluorescence-lifetime signals from quiescent and activated T cells can be used to classify the cells. Cancer exhibits altered cellular metabolism, which affects the autofluorescence of metabolic cofactors NAD (P)H and FAD. Fluorescence-guided surgery (FGS) is used to perform Keywords: Angiogenesis, Autofluorescence imaging, Multiband imaging, Narrow band imaging, Zoom endoscopy INTRODUCTION It is well known that angiogenesis is critical in the transition from premalignant to malignant lesions. Optical clearing of tissues is essential for Pavlova I, Williams M, EI-Naggar A, Richards-Kortum R, Gillenwater A. Autofluorescence properties of oral mucosa have been gaining interest in the field of early diagnosis of oral premalignant lesions. Autofluorescence-based tissue visualization, which is backed by over $50 million in research funded by the NIH and other respected organizations, has been used in other medical applications for over two decades. Fluorescence lifetime imaging microscopy (FLIM Sep 1, 2015 · Abstract Background: Oral cancer is a serious global issue and early diagnosis of oral cancer is the key in reducing the high mortality rate. In this review, the optical principles behind white-light and autofluorescence Abstract Autofluorescence imaging (AFI) is expected to better detect colorectal neoplasms than the conventional white light imaging (WLI). The use of these reagents is particularly suited to reduce the reversible Schiff's bases that are formed by the aldehyde-NH2 reaction and lead to autofluorescence, especially when using glutaraldehyde. Autofluorescence imaging and spectroscopy is a non-invasive, label-free, real-time technique for cancer detection Aug 29, 2011 · Image enhanced endoscopies, such as narrow band imaging (NBI) and autofluorescence imaging (AFI), were recently developed and clinically applied for the diagnosis of gastrointestinal diseases. Feb 3, 2025 · Request PDF | An outcome analysis of utilizing contrast-free near-infrared autofluorescence imaging in thyroid cancer surgery: a retrospective study | Hypoparathyroidism is the most common Jun 22, 2023 · Sorger and colleagues present Orion, a technique to analyze H&E histology and multiplex immunofluorescence imaging data from the same cells, which they apply to human colorectal cancer samples Autofluorescence imaging videobronchoscopy (AFI) is one of the new systems of autofluorescence bronchoscopy designed for thorough examination of bronchial mucosa. Direct visual inspection of tissue autofluorescence has shown encouraging results in high-prevalence populations, but the technique requires subjective interpretation and depends on the visual Aug 23, 2013 · The main criticism of autofluorescence in cancer diagnostostics was the lacking ability of discriminating high-risk from low-risk lesions [11]. Sep 27, 2020 · Background: Oral cancer screening is important for early detection and early treatment, which help improve survival rates. From the results of recent clinical studies, these novel technologies appear to be useful for detecting, as well as evaluating, gastrointestinal disorders. This study evaluated the efficacy of autofluorescence imaging based on the current standard oral mucosal disorder checklist in Taiwan. Based on the correlation between scar autofluorescence in AFI could diagnose the extent of CAFG as a green area in the gastric body, with higher reproducibility compared with white-light endoscopy. Autofluorescence When cells interact with the light of particular wavelength, they become excited and re-emit light of varying wavelength (color), which is called as autofluorescence. Carcinoma of unknown primary site presents with lymph node or distant metastasis, for which the site of primary tumour is not detectable. It’s caused primarily by metabolites and cellular structures that serve as natural fluorophores within the cell. Therefore, functional localization of the parathyroid glands during thyroid (parathyroid) surgery is a key focus and challenge in thyroid surgery. Optical imaging methods such as fluorescence imaging, Raman spectroscopy, diffuse optical tomography, and optical coherence tomography are emerging as promising structural and molecular imaging techniques for cancer diagnosis [2-6]. CSCs can be difficult to identify due to their small proportion of tumor and need for antibody-based labeling techniques. Autofluorescence is the natural fluorescence of biological structures such as mitochondria and lysosomes, in contrast to fluorescence originating from artificially added fluorescent markers (fluorophores). We present preliminary results of autofluorescence photodetection of cancerous oral mucosa. Aug 19, 2010 · Optical imaging of tissue autofluorescence has the potential to provide rapid label-free screening and detection of surface tumors for clinical applications, including when combined with endoscopy. Oral mucosal disorders detected by autofluoresce Mar 1, 2021 · Graphical abstract Autofluorescence and fluorescent probes allow the early detection of oral cancer through the loss of tissue autofluorescence or through the selective accumulation of fluorescent molecules in tumor cells after addition of fluorescent probes. A modified two-step desolvation method was employed to generate these nanoparticles whose size was controllable and had stable autofluorescence. These techniques are broadly discussed in this review. Yet, autofluorescence of the human brain and its neoplasia is sparsely examined. Methods A clinical CLE system with fiber probe and 488 nm laser excitation was used to acquire images of tissue autofluorescence. The purpose of the study is to establish a new model, help surgeons better identify, and protect PGs. Screening for NPC under conventional white light imaging (WLI) nasopharyngoscope examination remains a great clinical challenge due to its poor sensitivity. Recent advancements in TRFS technology, including the development of devices that simultaneously measure This clinical study is a first attempt to use autofluorescence for recurrence diagnosis of skin cancer in postoperative scars. Dec 5, 2018 · Oral cancer is a growing health issue in a number of low- and middle-income countries (LMIC), particularly in South and Southeast Asia. Here, we developed an integrated 4-modality endoscopy system combining WLI, autofluorescence imaging (AFI), diffuse Objective: This study set out to enhance the positive detection rate of lung tumors, diagnosis of nodular lesions and improve the accuracy of lung cancer classification by evaluating the clinical value of autofluorescence bronchoscopy (AFB). Sep 1, 2024 · Predicting DNA damage response in non-small cell lung cancer organoids via simultaneous label-free autofluorescence multiharmonic microscopy We report the clinical experiences of laparoscopic diagnosis with image enhancement and autofluorescence (AF) systems for laparoscopic gastric cancer surgery. Combination of narrow band imaging (NBI) and autofluorescence imaging (AFI) videobronchoscopy in endoscopic assessment of lung cancer extension. Modalities including autofluorescence bronchoscopy (AFB) and investigational tools targeted at detecting preinvasive lesions of the airway are discussed in this review. PpIX autofluorescence imaging is a simple and cost-effective cervical cancer screening method that could be performed during or after PDT to ensure effective treatment or remission as a change in Sep 13, 2023 · Here, we report the development of multi-modal autofluorescence and fluorescence imaging and spectroscopic (MAF-IS) smartphone-based systems for fast and real-time oral cancer screening. Jun 4, 2007 · This review focuses on our basic study results and clinical experience of fluorescence endoscopy for the gastrointestinal (GI) tract. The autofluorescence images demonstrated the cellular morphology and Nov 13, 2023 · Here, we used autofluorescence imaging of endogenous metabolic co-factors via multiphoton microscopy and FLIM in conjunction with oxygen consumption rate and extracellular acidification rate through Seahorse extracellular flux assays to detect changes in cellular metabolism in quiescent and classically activated macrophages in response to Abstract Purpose: Autofluorescence imaging is increasingly used to noninvasively identify neoplastic oral cavity lesions. In this review, we provide a comprehensive update on preclinical development and clinical applications of fluorescence imaging in the surgical management of liver cancers. Methodological quality was evalu … May 1, 2017 · It is common in VELscope® autofluorescence images that the intensity of the oral cancer region is lower than a normal region when the autofluorescence images for both buccal mucosa (Fig. Dec 1, 2020 · These results establish label-free autofluorescence imaging to quantify dynamic metabolism, polarization, and migration of macrophages at single-cell resolution within 3D microscale models. The validity of the method has been tested on 11 … Abstract Mitochondrial metabolism is an important contributor to cancer cell survival and proliferation that coexists with enhanced glycolytic activity. Global strategies targeted at lung cancer prevention and screening have been promoted to prevent or detect lung cancer in its early stages. Objective: To assess the impact of a novel, autofluorescence imaging (AFI) device on margin control for NMSCs before Mohs Jun 1, 2020 · Superficial location and infiltrative character of skin cancers make them a promising target for using optical imaging methods in their diagnostics. Mar 1, 2025 · Cancer is a major global health challenge, accounting for nearly one in six deaths worldwide. Native fluorescence, or autofluorescence (AF), consists in the emission of light in the UV-visible, near-IR spectral range when biological substrates are excited with light at suitable wavelength. Keywords: Narrow-band imaging, Autofluorescence imaging, I-scan, Flexible spectral imaging color enhancement, Confocal laser endomicroscopy INTRODUCTION The national cancer-screening program in South Korea has prioritized gastroenterologists' mission to diagnose and treat early gastrointestinal cancers. The purpose of the study is to establish a new model, help surgeon Lung cancer is one of the most common cancers, with high mortality rate worldwide. Jun 22, 2023 · We also explain the importance, value, and limitations of label-free autofluorescence imaging of NAD (P)H and FAD. Dec 1, 2015 · Abstract Subpopulations of cells that escape anti-cancer treatment can cause relapse in cancer patients. Autofluorescence imaging videobronchoscopy improves assessment of tumor margins and affects therapeutic strategy in central lung cancer. Here, we show that imaging of the autofluorescence lifetime signals of quiescent and activated T cells can be used to classify Nov 1, 2019 · Autofluorescence imaging (AFI) is an optical modality that can assist clinicians in detecting early cancers and high-grade dysplasia. D. This combined culture and imaging system provides unique insights into spatiotemporal tumor-immune cross-talk within the 3D TME. T cells isolated from human peripheral blood Fundus autofluorescence (FAF), a relatively new imaging modality, focuses on the fluorescent properties of pigments in the retina to generate images that help us view various disease processes from a different perspective. Various types of normal and malignant human tissue samples were utilized in this i … May 21, 2025 · High-Definition and Autofluorescence Bronchoscopic Imaging for Evaluating Epithelial Changes in Squamous Cell Lung Cancer After Neoadjuvant Immunochemotherapy: A Case Report Oct 28, 2009 · Background Autofluorescence imaging is used widely for diagnostic evaluation of various epithelial malignancies. Zaric B, Becker HD, Perin B, et al. Apr 23, 2016 · The aim of this prospective study was to evaluate the efficacy of a new form of autofluorescence imaging and tissue reflectance spectroscopy (Identafi, DentalEZ) in examining patients with oral mucosal lesions. We describe here the use of Subsequently, the Multimodal optical imaging- a combination of wide-field autofluorescence and high-resolution imaging may yield the best sensitivity and specificity for detection of oral cancer. Improving the diagnostic accuracy of these techniques requires a better understanding of the biological basis for optical changes associated with In order to improve the accuracy of endoscopic diagnosis of gastrointestinal tumors, especially early flat tumors, endoscopic autofluorescence imaging (AFI) as a new technique has been widely used in clinics in recent years. In this paper, we will give an overview of the literature on autofluorescence imaging and spectroscopy for various clinical questions. VELscope represents the first and most widely used oral application of this technology and has had numerous papers and case studies published supporting its ability to help discover May 5, 2009 · Autofluorescence imaging coupled with objective image analysis provided a sensitive and noninvasive tool for the detection of oral neoplasia. Fifty selected Squamous cell carcinoma. Diagnosis of larynx cancer relies on a detailed history and physical and objective assessment with endoscopy and imaging. Dec 6, 2009 · Autofluorescence imaging has shown high sensitivity for early diagnosis and detection of cancer in humans. Abstract Background and objective: An early detection of oral cancer might improve the patient's prognosis. The information from the Abstract Fibre-optic based time-resolved fluorescence spectroscopy (TRFS) is an advanced spectroscopy technique that generates sample-specific spectral-temporal signature, characterising variations in fluorescence in real-time. The proposed diagnostic parameter is based on a reduction in scar autofluorescence, evaluated in the green spectral channel. The aim of this study was to evaluate the impact of intraoperative parathyroid gland identification, using autofluorescence imaging, on the rate of post-operative (PO) hypoparathyroidism in thyroid cancer surgery. Jul 1, 2021 · Toluidine blue being an acidophilic dye, stains the acidic environment of tumor tissues [18]. Feb 1, 2024 · In comparison, multispectral autofluorescence (AF) imaging910 allows the distinction of several endogenous fluorophores at high spatial resolutions using relatively simple optical setups and linear unmixing techniques at scan speeds suitable for whole-slide imaging. Open in a new tab Feb 28, 2017 · Two-photon microscopy of cellular autofluorescence intensity and lifetime (optical metabolic imaging, or OMI) is a promising tool for preclinical drug development. The described dual-modality, dual-view, point-of-care oral cancer screening device, developed for high-risk populations in remote regions with limited infrastructure, implements autofluorescence imaging (AFI) and white light imaging (WLI) on a smartphone Autofluorescence is the natural fluorescence of biological structures such as mitochondria and lysosomes, in contrast to fluorescence originating from artificially added fluorescent markers (fluorophores). Abstract Background: We aimed to summarize the diagnostic accuracy of white light bronchoscopy (WLB) and advanced techniques for airway pre-cancerous lesions and early cancer, such as autofluorescence bronchoscopy (AFB), AFB combined with WLB (AFB + WLB) and narrow-band imaging (NBI) bronchoscopy. zka0 9ykcb jmd wnxof 2a ha2ee9 n3zx eivap lu k7icf